FastQCFastQC Report
Thu 13 Feb 2025
Bm88315_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBm88315_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7808561
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCATAGCTTATCTGGG814311.0428425928925957TruSeq Adapter, Index 11 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCATAGCTTATCTAGG762290.976223404030525TruSeq Adapter, Index 11 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCATAGCTTATCTAGT502480.6434988469706518TruSeq Adapter, Index 11 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCATAGCTTATCTGGT311750.3992412942666389TruSeq Adapter, Index 11 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)